9FW4
Crystal structure of Heme-Oxygenase mutant H20C from Corynebacterium diphtheriae complexed with Cobalt-porphyrine (HumO-Co(III))
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.744, 69.331, 63.177 |
| Unit cell angles | 90.00, 109.62, 90.00 |
Refinement procedure
| Resolution | 45.160 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.395 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.180 | 1.183 |
| Rpim | 0.093 | 0.620 |
| Number of reflections | 15723 | 1518 |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.991 | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 17% PEG10000, 100 mM BIS-TRIS pH 5.5, 100 mM ammonium acetate |
