9FW2

SARS CoV-2 nsp10 in complex with the ExoN domain from nsp14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	92.029, 104.782, 55.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.980 - 1.770
R-factor	0.1673
Rwork	0.167
R-free	0.18310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874:0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.150	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.189	1.629
Number of reflections	52881	2595
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.997	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.04-0.24 M Amino acids, 0.1 M buffer system 2 (pH 7.5), 16-38% v/v precipitant mix 2.