9FVC

Crystal structure of VcSiaP in complex with a VHH antibody (VHH_VcP#1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999
Spacegroup name	P 21 21 2
Unit cell lengths	169.607, 72.366, 85.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.180 - 2.640
R-factor	0.2229
Rwork	0.221
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.710
High resolution limit [Å]	2.640	2.640
Rmerge	0.097	0.530
Number of reflections	31540	2153
<I/σ(I)>	6.9
Completeness [%]	99.6
Redundancy	2
CC(1/2)	0.988	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	Tris BICINE, pH 8.5, PEG 550 MME, PEG 20000, Sodium fluoride, Sodium bromide, Sodium iodide