9FUD

Serial microseconds crystallography at ID29 using fixed-target (Si Chip): Lysozyme - without ligand GlcNAc (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-03-07
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.072
Spacegroup name	P 43 21 2
Unit cell lengths	78.700, 78.700, 37.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.650 - 2.050
R-factor	0.2197
Rwork	0.216
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.490
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.650	2.080
High resolution limit [Å]	2.050	2.050
Number of reflections	7923	323
<I/σ(I)>	4.16
Completeness [%]	100.0
Redundancy	101

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	293	1 M sodium acetate pH 3.0, 20% (w/v) NaCl, 5% (w/v) PEG 6000