9FTF
Crystal structure of the Connecting Domain of the Nipah virus Large protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.760, 50.540, 61.100 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 60.800 - 1.850 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.552 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 10.000 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.102 | |
| Number of reflections | 19413 | 19413 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 91.3 | 91.55 |
| Redundancy | 6 | |
| CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 35% tert-Butanol 0.1M tri-Sodium Citrate pH 5.6 |
