9FT8

Crystal structure of d(CGTGAATTCACG) with HT1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	294
Detector technology	PIXEL
Collection date	2022-08-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	2
Spacegroup name	P 21 21 21
Unit cell lengths	24.593, 40.406, 65.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.321 - 1.902
Rwork	0.219
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.809
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.321	1.951
High resolution limit [Å]	1.902	1.902
Number of reflections	5462	5200
<I/σ(I)>	4.36
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.964	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	10%MPD, 60mM MgCl2, 30 mM NaCaCo (pH6.8), 1mM Spermine