9FSV
Yeast 20S proteasome with human beta2i (1-53) in complex with epoxyketone inhibitor 42
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.700, 300.600, 144.480 |
Unit cell angles | 90.00, 112.83, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.750 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.24702 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.410 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.094 | 0.641 |
Number of reflections | 259664 | 26829 |
<I/σ(I)> | 7.5 | 2 |
Completeness [%] | 94.9 | |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |