9FSS
RNA Polymerase III Class III Open Mini Pre-Initiation complex 2 (OC2-mini)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.14 |
| Software | cryoSPARC,RELION,CTFFIND,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
