9FSA
Cell wall anchoring domain of the surface layer protein of Methanococcus voltae (aa 24-75; 484-576)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-06-12 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.3414 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 46.340, 53.268, 55.627 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.340 - 2.050 |
Rwork | 0.265 |
R-free | 0.28250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.075 |
Data reduction software | DIALS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.340 | 2.240 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.332 | 1.800 |
Number of reflections | 4977 | 291 |
<I/σ(I)> | 8.2 | 1.6 |
Completeness [%] | 90.7 | 70.1 |
Redundancy | 23.5 | 15.8 |
CC(1/2) | 0.996 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | JCSG+ eco condition C2 1.0 M LiCl, 0.1 M citrate pH 4.0, 20 % PEG 6000 0.3 ul condition + 0.3 ul protein solution |