9FS9
Self assembly domain of the surface layer protein of Viridibacillus arvi (aa 765-844)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-06-12 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.3414 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.017, 35.049, 68.017 |
Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
Resolution | 48.658 - 2.100 |
Rwork | 0.201 |
R-free | 0.25990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.386 |
Data reduction software | DIALS (3.8) |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.660 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.116 | 0.808 |
Number of reflections | 19156 | 1904 |
<I/σ(I)> | 9.3 | 1.4 |
Completeness [%] | 99.8 | |
Redundancy | 6 | |
CC(1/2) | 0.994 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | JCSG+ eco, condition G10 0.15 M KBr, 30 % PEG 2000 MME 22 g/l protein in 25 mM HEPES pH 7.5, 150 mM NaCl 0.3 ul condition + 0.3 ul protein |