9FS9

Self assembly domain of the surface layer protein of Viridibacillus arvi (aa 765-844)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-12
Detector	Bruker PHOTON III
Wavelength(s)	1.3414
Spacegroup name	P 1 21 1
Unit cell lengths	68.017, 35.049, 68.017
Unit cell angles	90.00, 91.23, 90.00

Refinement procedure

Resolution	48.658 - 2.100
Rwork	0.201
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.386
Data reduction software	DIALS (3.8)
Data scaling software	pointless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.660	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.116	0.808
Number of reflections	19156	1904
<I/σ(I)>	9.3	1.4
Completeness [%]	99.8
Redundancy	6
CC(1/2)	0.994	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	JCSG+ eco, condition G10 0.15 M KBr, 30 % PEG 2000 MME 22 g/l protein in 25 mM HEPES pH 7.5, 150 mM NaCl 0.3 ul condition + 0.3 ul protein