9FRR

Caspase recruitment domain (CARD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-15
Detector	RIGAKU HyPix-Arc 150
Wavelength(s)	1.34
Spacegroup name	P 65
Unit cell lengths	58.414, 58.414, 122.111
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.569 - 1.600
R-factor	0.1854
Rwork	0.184
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.658
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.569	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.061	0.512
Rpim	0.015	0.135
Number of reflections	31052	3119
<I/σ(I)>	35.31	5.66
Completeness [%]	99.9	99.97
Redundancy	17.1
CC(1/2)	1.000	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	0.9M Sodium citrate trihydrate 0.05M BIS-TRIS propane pH=7