9FRC
Architectural insights into spatially distinct corrin and metal binding sites of CobN
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.320, 73.600, 86.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.050 - 2.580 |
| R-factor | 0.18789 |
| Rwork | 0.184 |
| R-free | 0.27526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.733 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.050 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.067 | 0.559 |
| Number of reflections | 13012 | 4484 |
| <I/σ(I)> | 18.6 | 2.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 273 | 0.2M Lithium sulfate, 30% PEG 4000, 0.1M Tris pH 8.5. |
