9FQO
Crystal structure of C0083 Fab targeting the oxidized macrophage migration inhibitory factor (oxMIF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.540, 66.360, 58.650 |
| Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
| Resolution | 41.930 - 1.700 |
| R-factor | 0.2151 |
| Rwork | 0.215 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.761 |
| High resolution limit [Å] | 1.136 | 1.700 |
| Rmerge | 0.175 | 1.647 |
| Rmeas | 0.190 | |
| Rpim | 0.073 | 0.673 |
| Number of reflections | 42855 | 4260 |
| <I/σ(I)> | 9.22 | 1.44 |
| Completeness [%] | 99.7 | 99.51 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.995 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.33 | 293.15 | 0.1M Bis-Tris, 25.545% (w/v) PEG 3350 0.2M MgCl2 |
