9FQ5
Crystal structure of phosphoserine phosphatase (SerB) from Brucella melitensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 143.100, 143.100, 143.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.900 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 1.967 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 1.388 |
| Rmeas | 0.071 | 1.405 |
| Rpim | 0.011 | 0.215 |
| Number of reflections | 38642 | 3834 |
| <I/σ(I)> | 36.54 | 2.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 41.7 | 42.4 |
| CC(1/2) | 1.000 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, sodium malonate, Bis-Tris propane |
