9FQ4
Glyceraldehyde 3-phosphate dehydrogenase A (GAPDHA) NADP holoenzyme, from Helicobacter pylori, with active site cysteine oxidised to sulfenic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.478, 96.196, 94.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.362 - 1.200 |
| Rwork | 0.120 |
| R-free | 0.13660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.963 |
| Data reduction software | xia2.multiplex |
| Data scaling software | xia2.multiplex |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.362 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.089 | 0.737 |
| Number of reflections | 120012 | 5198 |
| <I/σ(I)> | 22.2 | 2.9 |
| Completeness [%] | 98.8 | 87.7 |
| Redundancy | 23.9 | 10.9 |
| CC(1/2) | 1.000 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M NH4SO4, 0.1M Tris pH 8.5, 25% w/v PEG3350 |
