9FOW
GPR180 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000036 |
| Spacegroup name | P 64 |
| Unit cell lengths | 143.116, 143.116, 43.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.250 - 1.878 |
| R-factor | 0.2217 |
| Rwork | 0.221 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.942 | 1.974 |
| High resolution limit [Å] | 1.878 | 1.878 |
| Rmerge | 0.081 | 2.601 |
| Number of reflections | 37931 | 37873 |
| <I/σ(I)> | 18.8 | 1.4 |
| Completeness [%] | 90.3 | 32.9 |
| Redundancy | 18.6 | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% glycerol, 16% PEG 8K, 0.04M kaliumdihydrogenphosphate |
