9FOD

Glyceraldehyde 3-phosphate dehydrogenase A (GAPDHA) apoenzyme, from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	I 2 2 2
Unit cell lengths	87.938, 94.811, 95.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.360 - 1.444
Rwork	0.140
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.769
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.360	1.570
High resolution limit [Å]	1.440	1.440
Rmeas	0.088	1.838
Number of reflections	55539	2777
<I/σ(I)>	16.6
Completeness [%]	95.4	69.3
Redundancy	13.7	13.7
CC(1/2)	0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M NH4SO4, 0.1M Bis-Tris pH 6.5, 25% w/v PEG3350 300nl drop, mixed either 1:1 or 2:1