9FOA
Artificial photoenzyme with anthraquinone cofactor and wild type streptavidin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.490, 57.490, 183.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.850 - 1.360 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_5246) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.850 | 1.379 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.041 | 0.483 |
| Rmeas | 0.048 | 0.581 |
| Number of reflections | 33304 | 1640 |
| <I/σ(I)> | 16.5 | 2.6 |
| Completeness [%] | 98.3 | 99.3 |
| Redundancy | 3.7 | 3.2 |
| CC(1/2) | 0.998 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2M Ammonium sulfate, 20% w/v PEG 3350 |
