9FMO

Crystal structure of C. merolae LAMMER-like dual specificity kinase (CmLIK) kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	65.522, 70.393, 88.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.380 - 2.487
R-factor	0.2318
Rwork	0.230
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.487	2.490
Number of reflections	14748	2323
<I/σ(I)>	9.39	0.95
Completeness [%]	99.0
Redundancy	7.4
CC(1/2)	0.996	0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris pH 8.0, 20 % (v/v) ethanol, 30 % (w/v) ethylene glycol