9FMH

PsiM N247M in complex with SAH and norbaeocystin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-27
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.7293
Spacegroup name	P 21 21 21
Unit cell lengths	49.372, 78.543, 83.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.490 - 0.900
R-factor	0.1379
Rwork	0.138
R-free	0.14810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.406
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.490	0.932
High resolution limit [Å]	0.900	0.900
Number of reflections	238138	23091
<I/σ(I)>	11.31
Completeness [%]	99.0
Redundancy	13.5
CC(1/2)	0.997	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris HCl pH 8.5, 20% PEG 8000, 200 mM MgCl2