9FMB
Crystal structure of human IgD-Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.6888 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.825, 43.667, 121.470 |
| Unit cell angles | 90.00, 107.96, 90.00 |
Refinement procedure
| Resolution | 52.450 - 3.000 |
| R-factor | 0.2313 |
| Rwork | 0.228 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.670 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.450 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.142 | 0.504 |
| Number of reflections | 21769 | 3452 |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 15.5 | 15.8 |
| CC(1/2) | 0.962 | 0.349 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1M Bis-Tris pH5.5, 25% PEG 3350, 0.2M ammonium acetate |
