9FLI
The structure of GanA, the extracellular galactanase of G.proteiniphilus T-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-09 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 160.339, 160.339, 155.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.040 - 3.200 |
| R-factor | 0.2548 |
| Rwork | 0.253 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.040 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.430 | 0.870 |
| Rmeas | 0.460 | 0.950 |
| Rpim | 0.160 | 0.370 |
| Number of reflections | 32203 | 4495 |
| <I/σ(I)> | 3.7 | 1.6 |
| Completeness [%] | 95.1 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.920 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% PEG 2K and 0.1M Sodium citrate at pH 5.5 |
