9FLH
Crystal structure of the C-terminal domain of VldE from Streptococcus pneumoniae containing four zinc atoms at the binding site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 3 |
| Unit cell lengths | 56.861, 56.861, 32.334 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.360 - 1.500 |
| Rwork | 0.196 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.726 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.036 | 1.335 |
| Number of reflections | 18745 | 956 |
| <I/σ(I)> | 0.998 | 0.809 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM HEPES, pH 7.5, 50 mM CADMIUM SULFATE, 1 M SODIUM ACETATE |
