9FL2
Crystal structure of Oscillatoria princeps pyranose oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.971, 137.406, 143.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.280 - 2.640 |
| R-factor | 0.19086 |
| Rwork | 0.188 |
| R-free | 0.25018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.616 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.285 | 2.868 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmeas | 0.380 | |
| Rpim | 0.106 | 0.727 |
| Number of reflections | 38297 | 1915 |
| <I/σ(I)> | 7.3 | 1.5 |
| Completeness [%] | 88.6 | 63.7 |
| Redundancy | 12.8 | |
| CC(1/2) | 0.941 | 0.296 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | PEG3350, sodium sulfate, bis-tris propane |
