9FKO
Arm segment of bacteriophage phi812
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.82330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.930, 75.140, 94.450 |
| Unit cell angles | 90.00, 109.87, 90.00 |
Refinement procedure
| Resolution | 46.490 - 1.800 |
| R-factor | 0.2467 |
| Rwork | 0.245 |
| R-free | 0.27530 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.490 | 1.860 |
| High resolution limit [Å] | 1.797 | 1.800 |
| Rmerge | 0.146 | 0.520 |
| Number of reflections | 109412 | 8201 |
| <I/σ(I)> | 3.04 | |
| Completeness [%] | 96.1 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 1.6 M LiCl, 0.1 M Tris (pH=7.5), 25 % w/v PEG 4 000 |
