9FK7
The structure of XT6 from G.proteiniphilus T-6: The E265G/N158E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.000, 61.915, 110.912 |
| Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
| Resolution | 35.890 - 2.200 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.824 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.890 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.370 |
| Rmeas | 0.100 | 0.460 |
| Rpim | 0.050 | 0.250 |
| Number of reflections | 29762 | 2566 |
| <I/σ(I)> | 11.8 | 2.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 4 | |
| CC(1/2) | 0.990 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 5K, 0.1M MES buffer at pH 6.5 and 0.01M Zinc chloride |
