9FK3

The structure of glycosynthase XT6 (E265G mutant), the extracellular xylanase of G.proteiniphilus T-6 in complex with xylobiose-F and xylotetraose-F molecules

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	I 1 2 1
Unit cell lengths	89.592, 61.901, 110.647
Unit cell angles	90.00, 105.25, 90.00

Refinement procedure

Resolution	34.100 - 2.200
R-factor	0.1567
Rwork	0.154
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.848
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.100	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.110	0.450
Rmeas	0.120	0.560
Rpim	0.060	0.310
Number of reflections	29862	2589
<I/σ(I)>	9.3	2.4
Completeness [%]	99.9
Redundancy	4
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.01M Zinc chloride, 17% PEG 5000, and 0.1M MES buffer at pH 6.5.