9FK1

The structure of glycosynthase XT6 (E265G mutant), the extracellular xylanase of G.proteiniphilus T-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	I 1 2 1
Unit cell lengths	88.980, 61.900, 111.322
Unit cell angles	90.00, 104.98, 90.00

Refinement procedure

Resolution	39.830 - 1.950
R-factor	0.1653
Rwork	0.163
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.830	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.090	0.800
Rmeas	0.100	0.930
Rpim	0.040	0.450
Number of reflections	42630	2952
<I/σ(I)>	16.6	1.6
Completeness [%]	99.6
Redundancy	5.6
CC(1/2)	0.990	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.01M Zinc chloride, 17% PEG 5000, and 0.1M MES buffer at pH 6.5.