9FJY
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 2).
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 0.01 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.79 |
Number of Particle | 4418492 |
Software | crYOLO,Coot,PHENIX,cryoSPARC,EPU,CTFFIND,UCSF ChimeraX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |