9FJ4
Structure of ubiquitin bound to coiled coil-UIM form 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.360, 49.710, 42.290 |
| Unit cell angles | 90.00, 123.78, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.540 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.777 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.590 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.026 | 0.724 |
| Number of reflections | 15709 | 1340 |
| <I/σ(I)> | 12.9 | 3.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M amino acids, 0.1 M buffer system 1, pH 6.6-6.7 and 36-38 %(v/v) precipitant mix 1 (Molecular Dimension) |
