9FIY

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH LRP6 INTERNALIZATION PEPTIDE SYRHFA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.928190
Spacegroup name	P 64
Unit cell lengths	128.431, 128.431, 74.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.720 - 2.880
R-factor	0.19634
Rwork	0.194
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.583
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	3.040
High resolution limit [Å]	2.880	2.880
Rmerge	0.086	1.630
Rmeas	0.091	1.700
Rpim	0.020	0.380
Number of reflections	16019	2322
<I/σ(I)>	23.8
Completeness [%]	99.9
Redundancy	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	2.0-2.2 M NaCl 0.4M Na/K phosphate 0.1M MES PH 7.1 15-20% glycerol