9FIX

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH LRP6 INTERNALIZATION PEPTIDE SYRPYSYRHFA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9677
Spacegroup name	P 64
Unit cell lengths	125.796, 125.796, 73.766
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.910 - 2.780
R-factor	0.19843
Rwork	0.197
R-free	0.23342
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.884
Data reduction software	xia2
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.910	2.930
High resolution limit [Å]	2.780	2.780
Rmerge	0.110	1.750
Rmeas	0.110	1.840
Rpim	0.035	0.560
Number of reflections	16878	2454
<I/σ(I)>	19.8	2.1
Completeness [%]	99.7
Redundancy	20.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	2.0-2.2 M NaCl 0.4M Na/K phosphate 0.1M MES PH 7.1 15-20% glycerol