9FIK

Structure of the FAB fragment of the Antibody NTF30037 in complex with NTF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.8856
Spacegroup name	C 2 2 21
Unit cell lengths	69.746, 108.549, 208.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.680 - 1.860
R-factor	0.1862
Rwork	0.186
R-free	0.18620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.680	2.032
High resolution limit [Å]	1.859	1.859
Number of reflections	51862	2593
<I/σ(I)>	9.3
Completeness [%]	92.2
Redundancy	13.4
CC(1/2)	0.996	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	15 % w/v Polyethylene glycol 8,000, 0.2 M Sodium Acetate, 0.1 M MES pH 6.5