9FI2
Bacteroides ovatus polysaccharide lyase family 38 (BoPL38) mutant N242A at pH 3.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03320 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 198.569, 89.146, 147.479 |
Unit cell angles | 90.00, 120.45, 90.00 |
Refinement procedure
Resolution | 49.160 - 2.570 |
R-factor | 0.2108 |
Rwork | 0.208 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.970 |
Data reduction software | XDS (jan 10, 2022) |
Data scaling software | XSCALE (jan 10, 2022) |
Phasing software | PHASER (1.21_5207) |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.160 | 2.600 |
High resolution limit [Å] | 2.570 | 2.570 |
Number of reflections | 70339 | 2471 |
<I/σ(I)> | 7.63 | |
Completeness [%] | 99.1 | |
Redundancy | 4.7 | |
CC(1/2) | 0.995 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294.15 | 18% (W/V) PEG3350, 0.2 M (NH4)2SO4 and 0.1 M citric acid pH 3.5 |