9FHZ
Bacteroides ovatus polysaccharide lyase family 38 (BoPL38) wild type in complex unsaturated guluronic acid tetramer at pH 3.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.421, 88.652, 147.307 |
| Unit cell angles | 90.00, 120.42, 90.00 |
Refinement procedure
| Resolution | 52.940 - 1.880 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.982 |
| Data reduction software | xia2 (3.12) |
| Data scaling software | xia2 (3.12) |
| Phasing software | PHASER (1.21_5207) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.940 | 5.100 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 174260 | 9217 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 97.1 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.992 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294.15 | 18% (W/V) PEG3350, 0.2 M (NH4)2SO4 and 0.1 M citric acid pH 3.5 |
