9FHT
Bacteroides ovatus polysaccharide lyase family 38 (BoPL38) wild type in complex hexaguluronic acid at pH 3.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.210, 88.850, 146.580 |
| Unit cell angles | 90.00, 120.36, 90.00 |
Refinement procedure
| Resolution | 42.540 - 2.050 |
| R-factor | 0.2358 |
| Rwork | 0.234 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.001 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | XSCALE (Jan 10, 2022) |
| Phasing software | PHASER (1.21_5207) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.540 | 2.070 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 279051 | 9223 |
| <I/σ(I)> | 9.04 | |
| Completeness [%] | 98.1 | |
| Redundancy | 1.014 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294.15 | 18% (W/V) PEG3350, 0.2 M (NH4)2SO4 and 0.1 M citric acid pH 3.5 |
