9FHS

Structure of the F13 protein of Vaccinia virus (F432 crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	F 4 3 2
Unit cell lengths	280.363, 280.363, 280.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.560 - 2.820
R-factor	0.2049
Rwork	0.203
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	2.950
High resolution limit [Å]	2.820	2.820
Number of reflections	43874	5510
<I/σ(I)>	13.96
Completeness [%]	96.3
Redundancy	43.5
CC(1/2)	0.999	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1 M Na3 citrate, 0.1 M Imidazole pH 8 (crystals cryoprotected in 33% glycerol)