9FHC

Crystallographic structure of AcrB V612F with bound minocycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-30
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	I 2 3
Unit cell lengths	227.460, 227.460, 227.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 2.200
Rwork	0.208
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	93149	6554
<I/σ(I)>	27.16
Completeness [%]	94.5
Redundancy	24.9
CC(1/2)	0.999	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	0.1M sodium acetate pH 4.5, 3-7% PEG200, 15-25% PEG400, 0.15M MgCl2, 0.15M NaCl