9FH7
OYE2 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 99.745, 101.265, 160.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.228 - 1.529 |
| R-factor | 0.17799 |
| Rwork | 0.177 |
| R-free | 0.20249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS (20180808) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.228 | 1.555 |
| High resolution limit [Å] | 1.529 | 1.529 |
| Rmerge | 0.120 | 1.255 |
| Rmeas | 0.136 | 1.429 |
| Rpim | 0.063 | 0.673 |
| Number of reflections | 122425 | 6095 |
| <I/σ(I)> | 9.9 | 1.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.6 | 4.5 |
| CC(1/2) | 0.997 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1 M sodium citrate, pH 5, 16% (v/v) PEG 10 000 |
