9FGP

cilia and flagella associated protein 299

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	31.756, 34.449, 58.984
Unit cell angles	91.35, 100.00, 90.09

Refinement procedure

Resolution	58.071 - 1.490
Rwork	0.174
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.962
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.071	1.520
High resolution limit [Å]	1.490	1.490
Number of reflections	28810	53
<I/σ(I)>	1.29
Completeness [%]	71.6
Redundancy	3.5
CC(1/2)	0.998	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20% PEG6000 -- 10% ethylene glycol -- 0.1M MES pH 6.0 -- 0.01M zinc chloride