9FGO
Crystal structure of Enterovirus 71 2A protease mutant C110A containing VP1-2A junction in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 61 |
| Unit cell lengths | 61.544, 61.544, 78.932 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.690 - 1.430 |
| R-factor | 0.15203 |
| Rwork | 0.151 |
| R-free | 0.16478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.882 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.056 | 0.726 |
| Rmeas | 0.057 | 0.773 |
| Rpim | 0.013 | 0.262 |
| Total number of observations | 12681 | |
| Number of reflections | 31516 | 1500 |
| <I/σ(I)> | 30.2 | 3 |
| Completeness [%] | 99.8 | |
| Redundancy | 18.8 | 8.5 |
| CC(1/2) | 1.000 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1M Sodium chloride, 15% ethanol |
