9FGI

LSSmOrange (P1) - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	51.260, 51.390, 195.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 1.770
R-factor	0.20041
Rwork	0.199
R-free	0.23958
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.128
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.770	5.280	1.770
Rmerge	0.093	0.032	2.740
Rmeas	0.108	0.036	2.330
Number of reflections	51138	2131	7970
<I/σ(I)>	16.6
Completeness [%]	99.1
Redundancy	13.1
CC(1/2)	0.998	0.999	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	20 % w/v Polyethylene glycol 6,000 100 mM tri-Sodium citrate