9FFI

mScarlet (SG C5) - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91814
Spacegroup name	C 1 2 1
Unit cell lengths	83.892, 45.098, 58.294
Unit cell angles	90.00, 101.78, 90.00

Refinement procedure

Resolution	41.100 - 1.500
R-factor	0.19142
Rwork	0.190
R-free	0.22352
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.868
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.065	0.027	1.260
Rmeas	0.076	0.031	1.471
Number of reflections	34204	404	2515
<I/σ(I)>	10.92
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.999	0.999	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	35 % v/v Polyethylene glycol 400 70 mM Sodium acetate 140 mM Lithium sulfate