9FFE
Carbohydrate active oxidoreductases from Phytophthora sojae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.045, 50.500, 53.092 |
| Unit cell angles | 88.10, 68.13, 74.28 |
Refinement procedure
| Resolution | 25.870 - 2.120 |
| R-factor | 0.1762 |
| Rwork | 0.174 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.897 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.420 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 22006 | 1603 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 97.3 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.987 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 2.0 M Ammonium sulfate, 0.1 M Bis-Tris pH 5.5 |
