9FFE

Carbohydrate active oxidoreductases from Phytophthora sojae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	43.045, 50.500, 53.092
Unit cell angles	88.10, 68.13, 74.28

Refinement procedure

Resolution	25.870 - 2.120
R-factor	0.1762
Rwork	0.174
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.897
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.420	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	22006	1603
<I/σ(I)>	6
Completeness [%]	97.3
Redundancy	6.6
CC(1/2)	0.987	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2.0 M Ammonium sulfate, 0.1 M Bis-Tris pH 5.5