9FF9
Crystal structure of N-terminal acetylated tropomyosin Cdc8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.401, 38.318, 97.542 |
| Unit cell angles | 97.71, 94.71, 101.50 |
Refinement procedure
| Resolution | 37.100 - 2.195 |
| R-factor | 0.263 |
| Rwork | 0.260 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.20.1_4487:) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.546 |
| High resolution limit [Å] | 2.195 | 2.195 |
| Rmerge | 0.068 | 0.515 |
| Rpim | 0.043 | 0.367 |
| Number of reflections | 6580 | 329 |
| <I/σ(I)> | 8 | 2.2 |
| Completeness [%] | 78.0 | 51.3 |
| Redundancy | 3.3 | 2.6 |
| CC(1/2) | 0.998 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Tris pH 8.0 43% MPD 350 mM ammonium acetat |
