9FF4

The structure of G.kaustophilus T-1 ScoC-17bp dsDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	68.634, 70.546, 95.468
Unit cell angles	89.77, 81.63, 60.91

Refinement procedure

Resolution	48.480 - 2.800
R-factor	0.2312
Rwork	0.228
R-free	0.29310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.191
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.660
Rmeas	0.090	0.770
Rpim	0.050	0.390
Number of reflections	37515	4515
<I/σ(I)>	6.3	1.6
Completeness [%]	98.3
Redundancy	3.8
CC(1/2)	0.930	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 3350 and 0.3 M Magnesium formate dihydrate