9FEO
LSSmOrange (P1) - Directionality of Optical Properties of Fluorescent Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-20 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.541870 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.955, 51.536, 53.892 |
| Unit cell angles | 95.69, 90.01, 105.68 |
Refinement procedure
| Resolution | 26.320 - 1.900 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.26201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.897 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.407 |
| Rmeas | 0.094 | 0.537 |
| Rpim | 0.046 | 0.348 |
| Total number of observations | 2036 | |
| Number of reflections | 33363 | 1309 |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 91.4 | |
| Redundancy | 3.7 | 1.6 |
| CC(1/2) | 0.996 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 100mM MES, 5 % w/v Polyethylene glycol 8000 40 % v/v 2-Methyl-2,4-pentanediol |
