9FE4
Crystallographic structure of AcrB V612F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 134.410, 134.410, 190.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.700 - 2.800 |
| Rwork | 0.259 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 49616 | 4859 |
| <I/σ(I)> | 20.19 | |
| Completeness [%] | 99.7 | |
| Redundancy | 11 | |
| CC(1/2) | 0.999 | 0.335 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.1M citrate pH 4.6, 5% PEG400, 16-21% PEG300, 8-11% glycerol |
