9FCY
CysG(N-16)-R21K mutant in complex with SAH from Kitasatospora cystarginea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.970, 106.170, 99.070 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.15105 |
| Rwork | 0.149 |
| R-free | 0.19613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.057 | 0.608 |
| Number of reflections | 87037 | 12753 |
| <I/σ(I)> | 12.3 | 2.4 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 0.1 M HEPES; 0.2 M NaCl; 20% PEG 4000 |
