9FCV
Cas nuclease-CRISPR (cr)RNA ribonucleoprotein (RNP) complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.09 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
